Resources

 
 

12/19/11


Below are provided links containing files and data described in the manuscript by the lab detailing methods to use low coverage, whole genome sequencing to map and identify causative mutations in the zebrafish using bulk segregant analysis of F2 mutant progeny.


Due to the size of the files, we have provided access from a linked cloud server where compressed files can be individually downloaded. Please note the expected size of the files. Also, please note, while these files are provided, we take no responsibility to the action (or inaction) of individual computers in handling the datasets.


We will periodically update these files with comments and additions. If you use this data in your work, please make sure to cite the above reference in resulting publications.


Good luck and good hunting!


MB, KH, MH

Feb 23, 2012
UPDATE

Due to high interest in the datasets, the cloud server we had organized was unable (or unwilling) to handle the amount of traffic that occurred. We apologize if you were not able to get access to the datasets.

We would like to provide this data to the community. Thus, we have organized another server that should be more robust. Please, help out your fellow researchers by downloading the files on your computer, once preferably, to use on your personal machine. We hope that this makes the data accessible and open to the community at large. If you experience continued difficulty in accessing the data, please let us know.

Best

MH

Apr 2, 2012
UPDATE

We have updated the SNP database to be more user friendly with headers to identify different wildtype strains for identification.

--MH

 

1) SNPs identified in our analysis of whole genome sequence data of four

commonly used reference strains as well as five mutants


2) Detailed protocol and scripts for using whole genome sequence data to

map and identify mutations.


Protocol (pdf 442kb)

    >Scripts and associated text tiles (0.2GB)

       scripts and text files (0.17MB)

        SNP datasets (390MB)

    >Aligned sequences of wt strains

        AB            5.69GB

        TLF            4.7GB

        Tu(B)         6.16GB

        Tu(G)        4.71GB

        WIK(B)      5.12GB

        WIK(G)     4.6GB

 

GENOMIC RESOURCES SUPPORTING MANUSCRIPT:

     EFFICIENT MAPPING AND CLONING OF MUTATIONS IN

     ZEBRAFISH BY LOW COVERAGE WHOLE GENOME

     SEQUENCING. (2012) BOWEN ET AL. GENETICS

© Katrin Henke